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Grant support

We acknowledge support from the European Union?s Horizon 2020 research and innovation programme under Marie Sk?odowska-Curie grant agreement No. 675737 (DiviDE ITN network) to A.L.D., R.K., I.V., and T.M.-R. Research in the M?ller-Reichert laboratory is sup- ported by funds from the Deutsche Forschungsgemeinschaft (MU 1423/8-2) . Work in the Vernos lab was supported by Spanish Ministry of Economy (MINECO) I +D grant BFU2012-37163 and BFU2015- 68726-P. A.L.D also received an EMBO short-term fellowship to visit the Muller-Reichert lab, grant agreement No. 8704. We thank Tobias F?rstenhaupt (Electron Microscopy Facility at the MPI-CBG, Dresden, Germany) for technical support. We also thank the Vernos and M?ller-Reichert groups and the members of the DiviDE ITN for discussions. We acknowledge the support of the Spanish Ministry of Economy, Industry and Competitiveness (MEIC) to the CRG-EMBL partnership and support of the Spanish Ministry of Economy and Competitiveness, ?Centro de Excelencia Severo Ochoa,? as well as support of the CERCA Programme/Generalitat de Catalunya.?

Analysis of institutional authors

Laguillo-Diego, AlejandraAuthorVernos, IsabelleCorresponding Author

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February 13, 2023
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MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles

Publicated to:Molecular Biology Of The Cell. 34 (1): - 2023-01-01 34(1), DOI: 10.1091/mbc.e22-08-0306-t

Authors: Laguillo-Diego, A; Kiewisz, R; Marti-Gómez, C; Baum, D; Müller-Reichert, T; Vernos, I

Affiliations

Barcelona Inst Sci & Technol, Ctr Genom Regulat, Barcelona 08003, Spain - Author
Cold Spring Harbor Lab, Simons Ctr Quantitat Biol, Cold Spring Harbor, NY 11724 USA - Author
Ctr Nacl Biotecnol CNB CSIC, Biocomp Unit, Darwin, Campus Univ Autonoma 3, Madrid 28049, Spain - Author
ICREA, Barcelona 08010, Spain - Author
New York Struct Biol Ctr, Simons Machine Learning Ctr, New York, NY 10027 USA - Author
Tech Univ Dresden, Fac Med Carl Gustav Carus, Expt Ctr, D-01307 Dresden, Germany - Author
Univ Pompeu Fabra, Barcelona 08003, Spain - Author
Weill Cornell Med, Sandra & Edward Meyer Canc Ctr, Sanford I Weill Dept Med, New York, NY 10021 USA - Author
Zuse Inst Berlin, Dept Visual & Data Ctr Comp, D-14195 Berlin, Germany - Author
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Abstract

Faithful chromosome segregation requires the assembly of a bipolar spindle, consisting of two antiparallel microtubule (MT) arrays having most of their minus ends focused at the spindle poles and their plus ends overlapping in the spindle midzone. Spindle assembly, chromosome alignment, and segregation require highly dynamic MTs. The plus ends of MTs have been extensively investigated but their minus-end structure remains poorly characterized. Here, we used large-scale electron tomography to study the morphology of the MT minus ends in three dimensionally reconstructed metaphase spindles in HeLa cells. In contrast to the homogeneous open morphology of the MT plus ends at the kinetochores, we found that MT minus ends are heterogeneous, showing either open or closed morphologies. Silencing the minus end-specific stabilizer, MCRS1 increased the proportion of open MT minus ends. Altogether, these data suggest a correlation between the morphology and the dynamic state of the MT ends. Taking this heterogeneity of the MT minus-end morphologies into account, our work indicates an unsynchronized behavior of MTs at the spindle poles, thus laying the groundwork for further studies on the complexity of MT dynamics regulation.

Keywords

CellsDynamic instabilityElectron-microscopyKinetochoresMechanismMitosisMolecular architectureNucleationTomographyTubulin ring complex

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal MOLECULAR BIOLOGY OF THE CELL due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2023, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Molecular Biology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-08-02:

  • WoS: 3
  • Europe PMC: 1

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-02:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 9.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 10 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 10.6.
  • The number of mentions on the social network X (formerly Twitter): 14 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Germany; United States of America.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (LAGUILLO DIEGO, ALEJANDRA) and Last Author (VERNOS, ISABELLE).

the author responsible for correspondence tasks has been VERNOS, ISABELLE.