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Analysis of institutional authors

Kallin, EricAuthorDe Andrés-Aguayo LAuthorVaras FAuthorInfante JfAuthorGraf TCorresponding Author

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March 25, 2020
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Musashi 2 is a regulator of the HSC compartment identified by a retroviral insertion screen and knockout mice

Publicated to: Blood. 118 (3): 554-564 - 2011-07-21 118(3), DOI: 10.1182/blood-2010-12-322081

Authors:

de Andrés-Aguayo, L; Varas, F; Kallin, EM; Infante, JF; Wurst, W; Floss, T; Graf, T
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Affiliations

CRG, Differentiat & Canc Program, Barcelona 08003, Spain - Author
Differentiation and Cancer Program, Center for Genomic Regulation and UPF, Barcelona, Spain. - Author
Helmholtz Ctr Munich, Inst Dev Genet, Munich, Germany - Author
ICREA, Barcelona, Spain - Author
Tech Univ Munich, Munich, Germany - Author
UPF, Barcelona, Spain - Author
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Abstract

We used a retroviral integration screen to search for novel genes that regulate HSC function. One of the genes that conferred HSC dominance when overexpressed due to an adjacent retroviral insertion was Musashi 2 (Msi2), an RNA-binding protein that can act as a translational inhibitor. A gene-trap mouse model that inactivates the gene shows that Msi2 is more highly expressed in long-term (LT) and short-term (ST) HSCs, as well as in lymphoid myeloid primed progenitors (LMPPs), but much less in intermediate progenitors and mature cells. Mice lacking Msi2 are fully viable for up to a year or more, but exhibit severe defects in primitive precursors, most significantly a reduction in the number of ST-HSCs and LMPPs and a decrease in leukocyte numbers, effects that are exacerbated with age. Cell-cycle and gene-expression analyses suggest that the main hematopoietic defect in Msi2-defective mice is the decreased proliferation capacity of ST-HSCs and LMPPs. In addition, HSCs lacking Msi2 are severely impaired in competitive repopulation experiments, being overgrown by wild-type cells even when mutant cells were provided in excess. Our data indicate that Msi2 maintains the stem cell compartment mainly by regulating the proliferation of primitive progenitors downstream of LT-HSCs.
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Keywords

Binding protein musashiC-mycC/ebp-alphaExpressionGene-transferHematopoietic stem-cellsIn-vivoMammalian cnsProliferationRna

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal BLOOD due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2011, it was in position 2/68, thus managing to position itself as a Q1 (Primer Cuartil), in the category Hematology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-04:

  • WoS: 56
  • Scopus: 50
  • Europe PMC: 45
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-04:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 70.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 70 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 3.
  • The number of mentions on Wikipedia: 1 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Germany.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (DE ANDRES AGUAYO, LUISA IRENE) and Last Author (GRAF, THOMAS HEINER).

the author responsible for correspondence tasks has been GRAF, THOMAS HEINER.

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Awards linked to the item

This work was supported by the Ministerio de Ciencia e Inovacion (SAF2007-63058). F.V. was supported by a grant from Instituto Carlos III-FIS (the Spanish Ministry of Health). E. M. K. was supported by a European Molecular Biology Organization (EMBO) long-term fellowship.
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