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Grant support

European Union's Horizon 2020 Research and Innovation Programme [CellViewer No 686637 to M.L.,M.P.C.]; Ministerio de Economia y Competitividad [BFU2013-49867-EXP to M.L., M.P.C.]; Fundacio Cellex Barcelona (to M. L); European Union Seventh Framework Programme under the European Research Council Grants [337191-MOTORS to M. L.]; 'Severo Ochoa' Programme for Centres of Excellence in R&D [SEV-2015-0522 to M.L.]; Ministerio de Economia y Competitividad and FEDER Funds [BFU2014-54717-P, BFU2015-71984-ERC to M.P.C.];

Analysis of institutional authors

Neguembor, Maria V.AuthorAulicino, FAuthorGomez-Garcia, Pablo A.AuthorCosma, Maria P.Corresponding Author

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Article

(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes

Publicated to:Nucleic Acids Research. 46 (5): e30- - 2018-03-16 46(5), DOI: 10.1093/nar/gkx1271

Authors: Neguembor, MV; Sebastian-Perez, R; Aulicino, F; Cosma, MP; Gomez-Garcia, PA; Lakadamyali, M

Affiliations

Advanced Fluorescence Imaging And Biophysics. The Institute of Photonic Sciences - Author
Barcelona Inst Sci & Technol, Ctr Genom Regulat CRG, Barcelona 08003, Spain - Author
Barcelona Inst Sci & Technol, ICFO Inst Ciencies Foton, Castelldefels 08860, Barcelona, Spain - Author
ICREA, Pg Lluis Co 23, Barcelona 08010, Spain - Author
Univ Penn, Perelman Sch Med, Dept Physiol, Clin Res Bldg,415 Curie Blvd, Philadelphia, PA 19104 USA - Author
UPF, Dr Aiguader 88, Barcelona 08003, Spain - Author
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Abstract

CRISPR/dCas9-based labeling has allowed direct visualization of genomic regions in living cells. However, poor labeling efficiency and signal to -background ratio have limited its application to visualize genome organization using super resolution microscopy. We developed (Po)STAC (Polycistronic SunTAg modified CRISPR) by combining CRISPR/dCas9 with SunTag labeling and polycistronic vectors. (Po)STAC enhances both labeling efficiency and fluorescence signal detected from labeled loci enabling live cell imaging as well as super resolution fixed-cell imaging of multiple genes with high spatiotemporal resolution.

Keywords

AnimalsCell lineCells, culturedCrispr-cas systemsDnaGenesGenetic vectorsHek293 cellsHela cellsHumansIn situ hybridization, fluorescenceLociLuminescent measurementsLuminescent proteinsMiceMicroscopyProteinsReproducibility of resultsRnaShelterinTelomereTelomeresTime-lapse imaging

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Nucleic Acids Research due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2018, it was in position 14/298, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations from Scopus Elsevier, it yields a value for the Field-Weighted Citation Impact from the Scopus agency: 1.07, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 14, 2024)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-20, the following number of citations:

  • WoS: 32
  • Scopus: 34
  • Europe PMC: 24
  • OpenCitations: 37

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-20:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 88.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 88 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 16.1.
  • The number of mentions on the social network X (formerly Twitter): 31 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: United States of America.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Neguembor, Victoire) .

the author responsible for correspondence tasks has been COSMA, MARIA PIA.